It is used in the diagnosis of infectious diseases through identification of microbial pathogens in clinical material, including organisms that are difficult or impossible to culture either by their nature or because of prior treatment with antimicrobial agents. Annealing typically refers to heat treatment in which a cold-worked metal is softened by allowing it to recrystallize. A series of cycles involving heat-denaturation, primer annealing and DNA synthesis by primer extension results in the amplification of the target DNA at approximately 2n, where n is the number of cycles. WordGame. n. Abbr. The annealing process requires the material above its recrystallization temperature for a set amount of time before cooling. Nucleotide sequence analysis of 26 cloned PCR products showed that in PCRs with papA primers, six out of eight obtained PCR products were false due to non-specific binding of the forward primer on … Particle size remained nearly the same regardless of the, The degree of crystallization of LiNi[O.sub.2] was enhanced at 500[degrees]C because the (104) reflection became stronger as the, Grain size of polysilicon layer which directly affects the resistivity of the layer is controlled by deposition temperature, dopant concentration and, The reaction is now rapidly cooled to a temperature at or slightly below the, The eventual extent of deformation is a function of the, Systematic evaluation revealed that the terminator concentration and the, Dictionary, Encyclopedia and Thesaurus - The Free Dictionary, the webmaster's page for free fun content, Hole-Transporting Layer Treatment of Planar Hybrid n-Si/PEDOT:PSS Solar Cells with Power Conversion Efficiency up to 14.5%, Nanoscale Mechanical Properties of Nanoindented [Ni.sub.4.88][Mn.sub.27.2][Ga.sub.24] Ferromagnetic Shape Memory Thin Film, Thermal annealing of sequentially deposited SnS thin films/SnS kilede keemiline sadestamine ZnS ja CdS alusele ning nende jargnev termiline tootlemine, Fabrication of Ti[O.sub.2] nanofilm photoelectrodes on Ti foil by Ti ion implantation and subsequent annealing, Structural and electrochemical properties of lithium nickel oxide thin films, Low loss optimization of strip channel polysilicon waveguide fabrication, PCR: the basics of the polymerase chain reaction, Thermal-induced recovery of small deformations and degradation defects on epoxy coating surface, Slimmer nanorods may revolutionise 3-D computer chip technology, Mass spectrometry-based detection of hemoglobin E mutation by allele-specific base extension reaction, Anneau des Ressources Francophones en Education. ... films by annealing the amorphous samples and the average grain size was sequentially increased by further increasing the annealing temperature. annealing: The joining-up of complementary single strands of DNA that have been separated by heat, or the joining of parts of separate strands that have complementary base sequences, to form a double helix. Isothermal annealing. This can be beneficial for many reasons. Complete annealing takes a long time, especially when the austenitic steel is more stable. With Phire Hot Start DNA Polymerase, use primers with Tm 60°C or higher. Annealing involves heating steel to a specified temperature and then cooling at a very slow and controlled rate, whereas tempering involves heating the metal to a precise temperature below the critical point, and is often done in air, vacuum or inert atmospheres. annealing是将工件加热到适当温度，根据材料和工件尺寸采用不同的保温时间，然后进行缓慢冷却，目的是使金属内部组织达到或接近平衡状态，获得良好的工艺性能和使用性能，或者为进一步淬 … Annealing glass to its perfect stress relief point is called the “annealing point.” Reaching the annealing point is a delicate process and requires diligence because the ideal annealing temperature must be reached, and then the piece has to be heat-soaked until it has an even internal temperature. Finish Annealing: It is a low temperature annealing treatment (at about 510°C) applied to cold worked steels with low or medium carbon content. The annealing temperature is usually ranging from 55ºC to 65ºC. It is the most common of all heat-treatment processes. Essentially, annealing takes the best of each range of the temperature spectrum, leveraging high temperatures to solve the more broad parts of the problem (in a more inexact way), and low temperatures to drive toward a more exact solution (by focusing on the particular area identified while at high temperature). annealing-point temperature Chinese meaning, annealing-point temperature的中文，annealing-point temperature的中文，annealing-point temperature的中文，translation, pronunciation, synonyms and example sentences are provided by ichacha.net. 4. The chosen temperature depends on the strand-melting temperature of the primers and the desired specificity. 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